Phosphotungstic Acid Magnesium Precipitation Method
Phosphotungstic acid magnesium precipitation method is one of many method of precipitation, it uses sodium phosphotungstic acid and magnesium chloride as precipitants. According to different types of precipitants, the common seen methods are heparin - manganese precipitation, dextran sulfate - magnesium precipitation method, polyethylene glycol precipitation and phosphotungstic acid - magnesium precipitation method. Phosphotungstic acid - magnesium precipitation method can be used for determination of serum high-density lipoprotein cholesterol, high density lipoprotein serum separation.
Phosphotungstic acid magnesium precipitation method determination principle of serum high-density lipoprotein cholesterol: Serum lipoproteins can form insoluble complex with polanions and divalent metal ions, the formation is related to ratio of lipoprotein and lipoid. The smaller the ratio is, lipoprotein gets easier to precipitate. Choose phosphotungstic acid magnesium precipitation solution with certain concentration can precipitate CM, VLDL and LDL, after configuration the upper liquid only has HDL, use OPA-acetic acid-sulfuric acid colorimetric method to determine HDL-ch content in serum with dealt cholesterol standard solution.
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